Reporter

Part:BBa_K4799607:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtMRP3_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 915
    Illegal SpeI site found at 1878
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3687
    Illegal SpeI site found at 915
    Illegal SpeI site found at 1878
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1669
    Illegal BamHI site found at 1652
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 915
    Illegal SpeI site found at 1878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 915
    Illegal SpeI site found at 1878
    Illegal NgoMIV site found at 2102
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1902
    Illegal BsaI.rc site found at 2517
    Illegal SapI.rc site found at 2688


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References